Details of experiments between P0DTC9 and O95049
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: TJP3
Protein B fragment: 1-109
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: TJP3
Protein B fragment: 189-274
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.14
Holdup BI standard deviation: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: TJP3
Protein B fragment: 373-482
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.12
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.