Details of experiments between the fragments P0DTC4~66-75 and Q8N3R9~238-336
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SARS-CoV2-E
Protein A fragment: 66-75
Protein A site: PBM
Protein A sequence: NSSRVPDLLV
Protein A construct: biotin-NLNSSRVPDLLV
Protein B protein: MPP5
Protein B fragment: 238-336
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.01