Details of experiments between the fragments P0DTC3~266-275 and Q9Y6N9~75-178
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SARS-Cov2-3a
Protein A fragment: 266-275
Protein A site: PBM
Protein A sequence: EPTTTTSVPL
Protein A construct: Biotin-(PEG)3-YDEPTTTTSVPL
Protein B protein: USH1C
Protein B fragment: 75-178
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.2
Holdup BI standard deviation: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.