Details of experiments between P09619 and Q68DX3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDGFRB
Protein A fragment: 1097-1106
Protein A site: PBM
Protein A sequence: PRAEAEDSFL
Protein A construct: biotin-ado-ado-PRAEAEDSFL
Protein B protein: FRMPD2
Protein B fragment: 944-1038
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.52
Experiment series 2
Protein A protein: PDGFRB
Protein A fragment: 1097-1106
Protein A site: PBM
Protein A sequence: PRAEAEDSFL
Protein A construct: biotin-ado-ado-PRAEAEDSFL
Protein B protein: FRMPD2
Protein B fragment: 1073-1166
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.51
Experiment series 3
Protein A protein: PDGFRB
Protein A fragment: 1097-1106
Protein A site: PBM
Protein A sequence: PRAEAEDSFL
Protein A construct: biotin-ado-ado-PRAEAEDSFL
Protein B protein: FRMPD2
Protein B fragment: 770-861
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.