Details of experiments between P07550 and O43464
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ADRB2
Protein A fragment: 404-413
Protein A site: PBM
Protein A sequence: RNCSTNDSLL
Protein A construct: Biotin-Ahx-RNCSTNDSLL
Protein B protein: HTRA2
Protein B fragment: 355-458
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.