Details of experiments between the fragments P06427~140-149 and Q6P0Q8~1102-1193
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRSRRRETAL
Protein A construct: biotin-ttds-SRSRRRETAL
Protein B protein: MAST2
Protein B fragment: 1102-1193
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 6.16
Standard deviation of dissociation constant (10-6M): 0.48
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 5.21
Experiment series 2
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRSRRRETAL
Protein A construct: biotin-ttds-SRSRRRETAL
Protein B protein: MAST2
Protein B fragment: 1102-1193
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.32
Experiment series 3
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A modification: (TPO)147
Protein A sequence: SRSRRRETAL
Protein A construct: Biotin-ttds-SRSRRREpTAL
Protein B protein: MAST2
Protein B fragment: 1102-1193
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.62
Experiment series 4
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRSRRRETAL
Protein A construct: biotin-ttds-SRSRRRETAL
Protein B protein: MAST2
Protein B fragment: 1102-1193
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.8
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 20.2
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.37