Details of experiments between the fragments P06427~140-149 and Q13884~107-196
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRSRRRETAL
Protein A construct: biotin-ttds-SRSRRRETAL
Protein B protein: SNTB1
Protein B fragment: 107-196
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.85
Experiment series 2
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A modification: (TPO)147
Protein A sequence: SRSRRRETAL
Protein A construct: Biotin-ttds-SRSRRREpTAL
Protein B protein: SNTB1
Protein B fragment: 107-196
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Normalized holdup BI: -0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRSRRRETAL
Protein A construct: biotin-ttds-SRSRRRETAL
Protein B protein: SNTB1
Protein B fragment: 107-196
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.55
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.66
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 20.2
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.05