Details of experiments between P06427 and Q00013
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV33-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRSRRRETAL
Protein A construct: biotin-ttds-SRSRRRETAL
Protein B protein: MPP1
Protein B fragment: 69-153
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.79