Details of experiments between the fragments P06400~365-379 and P00519~56-121
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RB1
Protein A fragment: 365-379
Protein A site: PxxP_2
Protein A sequence: EVNVIPPHTPVRTVM
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: ABL1
Protein B fragment: 56-121
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.