Details of experiments between the fragments P03409~344-353 and Q13368~124-222
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HTLV1-TAX1
Protein A fragment: 344-353
Protein A site: PBM
Protein A sequence: SEKHFRETEV
Protein A construct: biotin-ttds-SEKHFRETEV
Protein B protein: MPP3
Protein B fragment: 124-222
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.