Details of experiments between the fragments P00519~56-121 and Q96HA1~166-180
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ABL1
Protein A fragment: 56-121
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: POM121
Protein B fragment: 166-180
Protein B site: PxxP_3
Protein B sequence: PPARPAPRSPPPRSP
Protein B construct: Bio Ado Ado-PPARPAPRSPPPRSP-CONH2
Average holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.59