Details of experiments between the fragments P00519~56-121 and Q8IZ21~161-175
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ABL1
Protein A fragment: 56-121
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PHACTR4
Protein B fragment: 161-175
Protein B site: PxxP_1
Protein B sequence: ENVPKPPLLPPKRPL
Protein B construct: Bio Ado Ado-ENVPKPPLLPPKRPL-CONH2
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.66