Details of experiments between O96018 and O75084
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A protein: APBA3
Protein A fragment: 390-479
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FZD7
Protein B fragment: 565-574
Protein B site: PBM
Protein B sequence: SHSSKGETAV
Protein B construct: biotin-ttds-SHSSKGETAV

Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.

Experiment series 2

Protein A protein: APBA3
Protein A fragment: 482-569
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FZD7
Protein B fragment: 565-574
Protein B site: PBM
Protein B sequence: SHSSKGETAV
Protein B construct: biotin-ttds-SHSSKGETAV

Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.

Experiment series 3

Protein A protein: APBA3
Protein A fragment: 390-479
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FZD7
Protein B fragment: 565-574
Protein B site: PBM
Protein B sequence: SHSSKGETAV
Protein B construct: biotin-ttds-SHSSKGETAV

Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.