Details of experiments between the fragments O75970~246-342 and Q92793~2433-2442
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MPDZ
Protein A fragment: 246-342
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CBP
Protein B fragment: 2433-2442
Protein B site: PBM
Protein B sequence: DTLEKFVEGL
Protein B construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.