Details of experiments between the fragments O75970~1858-1949 and Q9C0A0~1299-1308
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MPDZ
Protein A fragment: 1858-1949
Protein A site: PDZ_12
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CNTNAP4
Protein B fragment: 1299-1308
Protein B site: PBM
Protein B sequence: VNENQKEYFF
Protein B construct: Biotin-Ahx-VNENQKEYFF
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.