Details of experiments between O75970 and P03409
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MPDZ
Protein A fragment: 1982-2070
Protein A site: PDZ_13
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.5
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.77
Experiment series 2
Protein A protein: MPDZ
Protein A fragment: 1141-1246
Protein A site: PDZ_7
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.33
Holdup BI standard deviation: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.44
Experiment series 3
Protein A protein: MPDZ
Protein A fragment: 1722-1810
Protein A site: PDZ_11
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.25
Experiment series 4
Protein A protein: MPDZ
Protein A fragment: 1624-1716
Protein A site: PDZ_10
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.22
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.18
Experiment series 5
Protein A protein: MPDZ
Protein A fragment: 371-467
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.13
Holdup BI standard deviation: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.92
Experiment series 6
Protein A protein: MPDZ
Protein A fragment: 120-230
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: -0
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: MPDZ
Protein A fragment: 1858-1949
Protein A site: PDZ_12
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: -0.04
Holdup BI standard deviation: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: MPDZ
Protein A fragment: 246-342
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: -0.06
Holdup BI standard deviation: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: MPDZ
Protein A fragment: 548-634
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.1
Holdup BI standard deviation: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: MPDZ
Protein A fragment: 688-793
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: -0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: MPDZ
Protein A fragment: 998-1105
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.01
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: MPDZ
Protein A fragment: 1325-1441
Protein A site: PDZ_8
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.03
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: MPDZ
Protein A fragment: 1464-1572
Protein A site: PDZ_9
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.01
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: MPDZ
Protein A fragment: 1624-1716
Protein A site: PDZ_10
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: -0.12
Normalized holdup BI: -0.12
Immobilized partner concentration in holdup experiment (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: MPDZ
Protein A fragment: 1858-1949
Protein A site: PDZ_12
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: MPDZ
Protein A fragment: 688-793
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 17
Protein A protein: MPDZ
Protein A fragment: 1624-1716
Protein A site: PDZ_10
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.6
Experiment series 18
Protein A protein: MPDZ
Protein A fragment: 1858-1949
Protein A site: PDZ_12
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 19
Protein A protein: MPDZ
Protein A fragment: 688-793
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HTLV1-TAX1
Protein B fragment: 344-353
Protein B site: PBM
Protein B sequence: SEKHFRETEV
Protein B construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.