Details of experiments between the fragments O75112~1-85 and Q9NPC6~255-264
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LDB3
Protein A fragment: 1-85
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: MYOZ2
Protein B fragment: 255-264
Protein B site: PBM
Protein B sequence: DTTVPESEDL
Protein B construct: biotin-ado-ado-DTTVPESEDL
Average holdup BI: 0.73
Normalized holdup BI: 0.73
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.26