Details of experiments between the fragments O75112~1-85 and P0DTC4~66-75
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LDB3
Protein A fragment: 1-85
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-CoV2-E
Protein B fragment: 66-75
Protein B site: PBM
Protein B sequence: NSSRVPDLLV
Protein B construct: biotin-NLNSSRVPDLLV
Average holdup BI: -0.03
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: LDB3
Protein A fragment: 1-85
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-CoV2-E
Protein B fragment: 66-75
Protein B site: PBM
Protein B sequence: NSSRVPDLLV
Protein B construct: biotin-NLNSSRVPDLLV
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.