Details of experiments between the fragments O75084~565-574 and Q15700~189-283
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.39
Experiment series 2
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Normalized holdup BI: 0.41
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.55