Details of experiments between O75084 and Q86UL8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 11.42
Standard deviation of dissociation constant (10-6M): 0.8
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.94
Experiment series 2
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.98
Experiment series 3
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 1141-1229
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.85
Experiment series 4
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 915-1017
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.72
Experiment series 5
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 1-102
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.39
Experiment series 6
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 600-682
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.28
Experiment series 7
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 772-865
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.44
Normalized holdup BI: 0.48
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.67
Experiment series 9
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 1141-1229
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Normalized holdup BI: 0.35
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.43
Experiment series 10
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 600-682
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.49
Experiment series 11
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 772-865
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: MAGI2
Protein B fragment: 915-1017
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.