Details of experiments between O75084 and Q12923
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: PTPN13
Protein B fragment: 1085-1188
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: PTPN13
Protein B fragment: 1362-1455
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: PTPN13
Protein B fragment: 1495-1591
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: PTPN13
Protein B fragment: 1779-1873
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: PTPN13
Protein B fragment: 1875-1973
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: FZD7
Protein A fragment: 565-574
Protein A site: PBM
Protein A sequence: SHSSKGETAV
Protein A construct: biotin-ttds-SHSSKGETAV
Protein B protein: PTPN13
Protein B fragment: 1085-1188
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.