Details of experiments between O60759 and Q8TEW8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PSCDBP
Protein A fragment: 350-359
Protein A site: PBM
Protein A sequence: RAVEEEESRF
Protein A construct: Biotin-Ahx-RAVEEEESRF
Protein B protein: PARD3B
Protein B fragment: 184-299
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.06