Details of experiments between the fragments O60292~959-1043 and Q14CM0~1313-1322
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SIPA1L3
Protein A fragment: 959-1043
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.