Details of experiments between O60292 and P59637
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SIPA1L3
Protein A fragment: 959-1043
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov-E
Protein B fragment: 67-76
Protein B site: PBM
Protein B sequence: SSEGVPDLLV
Protein B construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: 0.06
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.