Details of experiments between the fragments O43516~304-318 and Q14155~178-251
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: WIPF1
Protein A fragment: 304-318
Protein A site: PxxP_7
Protein A sequence: SQAPPPPPPPSRPGP
Protein A construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Protein B protein: ARHGEF7
Protein B fragment: 178-251
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.56