Details of experiments between O43182 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGAP6
Protein A fragment: 965-974
Protein A site: PBM
Protein A sequence: NPDALPETLV
Protein A construct: biotin-ttds-NPDALPETLV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Normalized holdup BI: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.23
Experiment series 2
Protein A protein: ARHGAP6
Protein A fragment: 965-974
Protein A site: PBM
Protein A sequence: NPDALPETLV
Protein A construct: biotin-ttds-NPDALPETLV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Normalized holdup BI: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.01