Details of experiments between the fragments O43166~946-1030 and O75084~565-574
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SIPA1L1
Protein A fragment: 946-1030
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FZD7
Protein B fragment: 565-574
Protein B site: PBM
Protein B sequence: SHSSKGETAV
Protein B construct: biotin-ttds-SHSSKGETAV
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.