Details of experiments between the fragments O15439~1316-1325 and Q13796~22-109
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: SHROOM2
Protein B fragment: 22-109
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.44