Details of experiments between O15439 and Q8TBB1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 261-371
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.11
Experiment series 2
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 371-470
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 500-596
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.21
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 261-371
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.59
Experiment series 5
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 371-470
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 500-596
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 261-371
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.95
Experiment series 8
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 371-470
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.62
Experiment series 9
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: biotin-ttds-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 500-596
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.66
Experiment series 10
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: Biotin-ado-ado-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 371-470
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: Biotin-ado-ado-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 500-596
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: ABCC4
Protein A fragment: 1316-1325
Protein A site: PBM
Protein A sequence: STLTIFETAL
Protein A construct: Biotin-ado-ado-STLTIFETAL
Protein B protein: LNX1
Protein B fragment: 261-371
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.