Details of experiments between the fragments O15085~41-126 and Q9NR12~448-457
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF11
Protein A fragment: 41-126
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PDLIM7
Protein B fragment: 448-457
Protein B site: PBM
Protein B sequence: LCKSHAFSHV
Protein B construct: Biotin-Ahx-LCKSHAFSHV
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.48