Details of experiments between the fragments O15085~41-126 and Q8WWN8~1417-1431
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF11
Protein A fragment: 41-126
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARAP3
Protein B fragment: 1417-1431
Protein B site: Internal_PBM
Protein B construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Average holdup BI: -0.04
Normalized holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.