Details of experiments between O15056 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SYNJ2
Protein A fragment: 1-1496
Protein A construct: endogenous protein (Jurkat)
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.77
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 3.56
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.29.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 3))
Number of measurements: 3
Measured pKd: 5.53
Experiment series 2
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.37
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.55
Experiment series 8
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.84
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 5.56
Experiment series 9
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.58
Experiment series 16
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.86
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 5.64
Experiment series 17
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 18
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.85
Experiment series 19
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.65
Experiment series 20
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 21
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 22
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.64
Experiment series 23
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.47
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.73
Experiment series 24
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.8
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 5.43
Experiment series 25
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 26
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 27
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 28
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 29
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 30
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 31
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 32
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.61
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 5
Experiment series 33
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 34
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 35
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 36
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 37
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 38
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 39
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.45
Experiment series 40
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.79
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 5.57
Experiment series 41
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 42
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 43
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 44
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 45
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 46
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 47
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.49
Experiment series 48
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.7
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 5.58
Experiment series 49
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 50
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 51
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 52
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 53
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 54
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 55
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 56
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.6
Experiment series 57
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 58
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 59
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 60
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 61
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 62
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 63
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 64
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.59
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.96
Experiment series 65
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 66
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 67
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 68
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 69
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 70
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 71
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.37
Experiment series 72
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.75
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 5.62
Experiment series 73
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 74
Protein A protein: SYNJ2
Protein A fragment: 1098-1112
Protein A site: PxxP_1
Protein A sequence: VPNRPRPPQPPQRPP
Protein A construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 75
Protein A protein: SYNJ2
Protein A fragment: 1099-1113
Protein A site: PxxP_2
Protein A sequence: PNRPRPPQPPQRPPP
Protein A construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 76
Protein A protein: SYNJ2
Protein A fragment: 1140-1154
Protein A site: PxxP_3
Protein A sequence: QTARLLPGAPQQPPK
Protein A construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 77
Protein A protein: SYNJ2
Protein A fragment: 1143-1157
Protein A site: PxxP_4
Protein A sequence: RLLPGAPQQPPKART
Protein A construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 78
Protein A protein: SYNJ2
Protein A fragment: 1219-1233
Protein A site: PxxP_5
Protein A sequence: ETPQAPPLLPRRPPP
Protein A construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 79
Protein A protein: SYNJ2
Protein A fragment: 1227-1241
Protein A site: PxxP_6
Protein A sequence: LPRRPPPRVPAIKKP
Protein A construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.59
Experiment series 80
Protein A protein: SYNJ2
Protein A fragment: 1324-1338
Protein A site: PxxP_7
Protein A sequence: EAPPLVPKVPPRRKK
Protein A construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.81
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 5.45
Experiment series 81
Protein A protein: SYNJ2
Protein A fragment: 1442-1456
Protein A site: PxxP_8
Protein A sequence: RSPKRDPIDPVSAGA
Protein A construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.