Details of experiments between the fragments O15018~722-815 and P36809~144-153
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 722-815
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.78