Details of experiments between the fragments O15018~64-181 and Q6ZTQ3~360-369
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 64-181
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RASSF6
Protein B fragment: 360-369
Protein B site: PBM
Protein B sequence: KLVIKTETTV
Protein B construct: biotin-ttds-KLVIKTETTV
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.