Details of experiments between the fragments O15018~575-684 and A6NIM6~570-579
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 575-684
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.1