Details of experiments between the fragments O15018~326-424 and Q6UVK1~2313-2322
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 326-424
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CSPG4
Protein B fragment: 2313-2322
Protein B site: PBM
Protein B sequence: PALKNGQYWV
Protein B construct: biotin-ado-ado-PALKNGQYWV
Average holdup BI: 0.81
Normalized holdup BI: 0.81
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.45