Details of experiments between the fragments O15018~326-424 and Q15018~406-415
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 326-424
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABRAXAS2
Protein B fragment: 406-415
Protein B site: PBM
Protein B sequence: DPRNTQTSQI
Protein B construct: biotin-ttds-DPRNTQTSQI
Average holdup BI: 0.31
Normalized holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.44