Details of experiments between the fragments O15018~326-424 and P26554~142-151
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 326-424
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV51-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: RTRQRNETQV
Protein B construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: PDZD2
Protein A fragment: 326-424
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV51-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: RTRQRNETQV
Protein B construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.58