Details of experiments between the fragments O15018~2741-2839 and P60484~391-403
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 2741-2839
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PTEN
Protein B fragment: 391-403
Protein B site: PBM
Protein B sequence: EDQHTQITKV
Protein B construct: Biotin-ttds-PFDEDQHTQITKV
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
PUBMED ID of publication: 33382810
Number of measurements: 1
The affinity was below the detection threshold of the assay.