Details of experiments between the fragments O15018~2611-2718 and Q92793~2433-2442
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD2
Protein A fragment: 2611-2718
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CBP
Protein B fragment: 2433-2442
Protein B site: PBM
Protein B sequence: DTLEKFVEGL
Protein B construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.8