Details of experiments between the fragments O14907~7-124 and P22460~604-613
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TX1BP3
Protein A fragment: 7-124
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNA5
Protein B fragment: 604-613
Protein B site: PBM
Protein B sequence: CLDTSRETDL
Protein B construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.17
Experiment series 2
Protein A protein: TX1BP3
Protein A fragment: 7-124
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNA5
Protein B fragment: 604-613
Protein B site: PBM
Protein B sequence: CLDTSRETDL
Protein B construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.22
Normalized holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.28