Details of experiments between O14907 and Q8TAA9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TX1BP3
Protein A fragment: 7-124
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: VANGL1
Protein B fragment: 515-524
Protein B site: PBM
Protein B sequence: VLRLQSETSV
Protein B construct: biotin-ttds-VLRLQSETSV
Average holdup BI: 0.19
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.22
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.21