Details of experiments between the fragments O14745~10-98 and O15439~1316-1325
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NHERF1
Protein A fragment: 10-98
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.63
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.36