Details of experiments between the fragments O14640~248-337 and Q5KSL6~1262-1271
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DVL1
Protein A fragment: 248-337
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DGKK
Protein B fragment: 1262-1271
Protein B site: PBM
Protein B sequence: DPLTPSRSQL
Protein B construct: biotin-ttds-DPLTPSRSQL
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.