Details of experiments between the fragments O14640~248-337 and P26554~142-151
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DVL1
Protein A fragment: 248-337
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV51-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: RTRQRNETQV
Protein B construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.32
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.86