Details of experiments between the fragments O14578~2018-2027 and P26045~505-597
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CIT
Protein A fragment: 2018-2027
Protein A site: PBM
Protein A sequence: VNKVWDQSSV
Protein A construct: biotin-ttds-VNKVWDQSSV
Protein B protein: PTPN3
Protein B fragment: 505-597
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.1
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.78