Details of experiments between the fragments O00592~549-558 and Q13884~107-196
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PODXL
Protein A fragment: 549-558
Protein A site: PBM
Protein A sequence: DLDEEEDTHL
Protein A construct: biotin-ttds-DLDEEEDTHL
Protein B protein: SNTB1
Protein B fragment: 107-196
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.04
Normalized holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.