Details of experiments between O00592 and Q07157
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PODXL
Protein A fragment: 549-558
Protein A site: PBM
Protein A sequence: DLDEEEDTHL
Protein A construct: biotin-ttds-DLDEEEDTHL
Protein B protein: TJP1
Protein B fragment: 184-264
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: PODXL
Protein A fragment: 549-558
Protein A site: PBM
Protein A sequence: DLDEEEDTHL
Protein A construct: biotin-ttds-DLDEEEDTHL
Protein B protein: TJP1
Protein B fragment: 415-516
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.