Details of experiments between O00499 and O43516
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 1-503
Protein B construct: endogenous protein (Jurkat)
Average holdup BI: 0.77
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.91
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.29.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 3))
Number of measurements: 3
Measured pKd: 5.52
Experiment series 2
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.51
Experiment series 10
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.52
Experiment series 15
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.54
Experiment series 16
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.69
Experiment series 17
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.12
Experiment series 18
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 19
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 20
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 21
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 22
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 23
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 24
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 25
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 26
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 27
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 28
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 29
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 30
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 31
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.73
Experiment series 32
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.61
Experiment series 33
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: T532M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.11
Experiment series 34
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 35
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 36
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 37
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 38
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 39
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 40
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 41
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.56
Experiment series 42
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 43
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 44
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 45
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 46
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.68
Experiment series 47
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.01
Experiment series 48
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.82
Experiment series 49
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Q540H
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.25
Experiment series 50
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 51
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 52
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 53
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 54
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.56
Experiment series 55
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 56
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 57
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 58
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 59
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 60
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 61
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 62
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 63
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 64
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 65
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: D537V
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 66
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 67
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 68
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 69
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 70
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 71
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 72
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 73
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 74
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 75
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 76
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 77
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 78
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 79
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 80
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 81
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V583I
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.04
Experiment series 82
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.87
Experiment series 83
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.03
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 84
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 85
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 86
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 87
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 88
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 89
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 90
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 91
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 92
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 93
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 94
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.01
Experiment series 95
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 96
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 97
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: P551L
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.21
Experiment series 98
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 99
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 100
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 101
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 102
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 103
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 104
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 105
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 106
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: -0.16
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 107
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 108
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 109
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 110
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.5
Experiment series 111
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 112
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 113
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: F584S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 114
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 115
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 116
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 117
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 118
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 119
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 120
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 121
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 122
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 123
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 124
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 125
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 126
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 127
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 128
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 129
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: Y531S
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.54
Experiment series 130
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 131
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.08
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 132
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 133
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 134
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 135
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 136
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 137
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 138
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 139
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 140
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 141
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 142
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.83
Experiment series 143
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.8
Experiment series 144
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 145
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: R581C
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.19
Experiment series 146
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 138-152
Protein B site: PxxP_1
Protein B sequence: AKPFSPPSGPGRFPV
Protein B construct: Bio Ado Ado-AKPFSPPSGPGRFPV-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 147
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.05
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 148
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 165-179
Protein B site: PxxP_3
Protein B sequence: QRNRMPPPRPDVGSK
Protein B construct: Bio Ado Ado-QRNRMPPPRPDVGSK-CONH2
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 149
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 198-212
Protein B site: PxxP_4
Protein B sequence: PHNRGSPPVPGGPRQ
Protein B construct: Bio Ado Ado-PHNRGSPPVPGGPRQ-CONH2
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.49
Experiment series 150
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 247-261
Protein B site: PxxP_5
Protein B sequence: SNRPPLPPTPSRALD
Protein B construct: Bio Ado Ado-SNRPPLPPTPSRALD-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 151
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 152
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 304-318
Protein B site: PxxP_7
Protein B sequence: SQAPPPPPPPSRPGP
Protein B construct: Bio Ado Ado-SQAPPPPPPPSRPGP-CONH2
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 153
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 326-340
Protein B site: PxxP_8
Protein B sequence: SGNDETPRLPQRNLS
Protein B construct: Bio Ado Ado-SGNDETPRLPQRNLS-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 154
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 350-364
Protein B site: PxxP_9
Protein B sequence: SPGRSGPLPPPPSER
Protein B construct: Bio Ado Ado-SPGRSGPLPPPPSER-CONH2
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 155
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 359-373
Protein B site: PxxP_10
Protein B sequence: PPPSERPPPPVRDPP
Protein B construct: Bio Ado Ado-PPPSERPPPPVRDPP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 156
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 372-386
Protein B site: PxxP_11
Protein B sequence: PPGRSGPLPPPPPVS
Protein B construct: Bio Ado Ado-PPGRSGPLPPPPPVS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 157
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 390-404
Protein B site: PxxP_12
Protein B sequence: STSRALPATPQLPSR
Protein B construct: Bio Ado Ado-STSRALPATPQLPSR-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 158
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 393-407
Protein B site: PxxP_13
Protein B sequence: RALPATPQLPSRSGV
Protein B construct: Bio Ado Ado-RALPATPQLPSRSGV-CONH2
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.69
Experiment series 159
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 408-422
Protein B site: PxxP_14
Protein B sequence: DSPRSGPRPPLPPDR
Protein B construct: Bio Ado Ado-DSPRSGPRPPLPPDR-CONH2
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.91
Experiment series 160
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 411-425
Protein B site: PxxP_15
Protein B sequence: RSGPRPPLPPDRPSA
Protein B construct: Bio Ado Ado-RSGPRPPLPPDRPSA-CONH2
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.75
Experiment series 161
Protein A protein: BIN1
Protein A fragment: 513-593
Protein A site: SH3
Protein A modification: V566M
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 490-503
Protein B site: PxxP_16
Protein B sequence: RRERGAPPLPPIPR_
Protein B construct: Bio Ado Ado-RRERGAPPLPPIPR_-CONH2
Average holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.19