Details of experiments between the fragments O00192~953-962 and Q6P0Q8~1102-1193
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARVCF
Protein A fragment: 953-962
Protein A site: PBM
Protein A sequence: AKPQPVDSWV
Protein A construct: Biotin-Ahx-AKPQPVDSWV
Protein B protein: MAST2
Protein B fragment: 1102-1193
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.11
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.86